您的位置：首页 > 产品中心 > High Pure 组织RNA提取试剂盒
High Pure 组织RNA提取试剂盒
High Pure RNA Tissue Kit
| 产品编号： | 4157337 |
| 规格： | sufficient for 50 isolation(s), suitable for RT-PCR, suitable for Northern blotting |
| 包装规格： | 50 ISOLATIONS |
| 产品类别： | 进口试剂 |
| 品牌： | Roche |
| 优惠价： | 立即咨询 |
产品别名
High Pure 组织RNA提取试剂盒
High Pure RNA Tissue Kit
产品性质
| Quality Level【质量水平】 | 100 |
| usage【用途】 | sufficient for 50 isolation(s) |
| manufacturer/tradename | Roche |
| packaging【包装】 | kit of for 50 isolations |
| technique(s) | Northern blotting: suitable RT-PCR: suitable |
基本信息
| General description【一般描述】 | The High Pure RNA Tissue Kit is designed for the purification of total, intact RNA from tissue samples, free of any contaminating DNA. Low to medium throughput RNA isolation. Nucleic acids bind to the surface of the glass fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure Filter Tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA. High pure RNA isolation kit can rapidly isolate intact total RNA from a broad range of research sample materials, including cultured cells, mammalian blood, white blood cells (WBCs), yeast, and bacteria. It rapidly isolates total RNA from mammalian tissues such as mouse liver, spleen, lung, and heart. Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume. Sample Material: Solid tissue (e.g., mouse liver, spleen, lung, heart): 1 - 25 mg. Note: Sample size depends on the method used to prepare the tissue; larger samples (>10 mg) should be processed via rotor-stator homogenization. |
| Application【应用】 | The High Pure RNA Tissue Kit rapidly isolates total RNA from mammalian tissues such as mouse liver, spleen, lung, and heart. The isolated RNA can be used in many downstream applications:
|
| Features and Benefits【特点和优势】 |
|
| Components【组分】 |
|
| Quality【质量】 |
|
| Preparation Note【制备说明】 | Tissue samples are disrupted and homogenized in the presence of a chaotropic salt (guanidine HCL) that inactivates RNases. The homogenate is then applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, all nucleic acids bind to the glass fiber fleece, while contaminating substances (salts, proteins, and other cellular contaminants) do not. DNA in the preparation is digested with DNase I directly on the filter. Brief wash-and-spin steps readily remove the digested DNA fragments and other cellular contaminants. The remaining purified RNA is then eluted in a small volume of low-salt buffer. |
| Other Notes【其他说明】 | High Pure Technology and Silica Adsorption Kits For life science research only. Not for use in diagnostic procedures. 图1：不同均质化程序的比较。使用各种程序均质化小鼠肝脏。用高纯核糖核酸组织试剂盒从每种匀浆中纯化总核糖核酸。分离的核糖核酸的等分试样用特异性针对谷氨酸脱羧酶基因区域的引物进行反向转录。通过凝胶电泳分析cDNA产物。 泳道1：均质化：Ultra Turrax；产量：1.9 & # 956；g/mg组织；A 260/ A 280 ： 2.0 泳道2：均质化：电动一次性塑料杵；产量：3 & # 956；g/mg组织；A 260 /A 280 ： 2.0 泳道3：均质化：研钵和杵，20 G针头；产量：1.5 & # 956；g/mg组织；A 260 /A 280 ： 2.0 泳道4：均质化：手动一次性塑料杵，20 G针头；产量：1.8 & # 956；g/mg组织；A 260 /A 280 ： 2.0 泳道5：均质化：手动一次性塑料杵；产量：3.4 & # 956；g/mg组织；A 260 /A 280 ： 2.0 泳道6：均质化：小珠涡旋；产量：3 & # 956；g/mg组织；A 260 /A 280 ： 2.0 泳道7：分子量标记 |
安全信息
| Pictograms【象形图】 | GHS07,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H302 + H332 - H315 - H317 - H319 - H334 |
| Precautionary Statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| Hazard Classifications【危险分类】 | Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
