IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant

100

≥97% (SDS-PAGE)

solid

Calbiochem^®

OK to freeze

<1.0 EU/μg protein Endotoxin

ambient

−20℃

25 μg in Glass bottle

Recombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.

Functional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.

Toxicity: Standard Handling (A)

Lyophilized from 0.2 µm sterile-filtered PBS.

Reconstitute to 100 µg/ml in sterile PBS.

Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun.310, 612.
Nagakubo, D. et al. 2003. J. Immunol.171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ.13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747.
Kato, M.V. 2000. Mol. Med.6, 126.
Hwa, V. et al. 1999. Endocr. Rev.20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981.
Kato, M.V. et al. 1996. Oncogene12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1