Immobilon^®-FL PVDF Transfer Membrane

32031602

NB.22

The Immobilon^®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. The Immobilon^®-FL membrane has a nominal pore size of 0.45 μm and is recommended for blotting proteins with molecular weights greater than 20 kilodaltons (kDa). The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection.
Filter Code: IPFL

Immobilon^®-FL Transfer Membrane has been used in western blotting.

Immobilon^®-FL Transfer Membrane:

• Designed for optimal fluorescent immunodetection application
• Extremely low background improves the sensitivity of all fluorescence based immunodetection
• Compatible with all commonly used fluorescent probes across a wide range of excitation/emission wavelengths
• For standard chemiluminescent or chromogenic detection

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

PVDF membrane
plain filter
white filter

400

hydrophobic

Immobilon^®

western blot: suitable

7 cm × 8.4 cm

0.45 μm pore size

155 μg/cm² adsorption capacity (insulin)
205 μg/cm² adsorption capacity (BSA)
300 μg/cm² adsorption capacity (goat IgG)

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

chemiluminescent
colorimetric
fluorometric

ambient

11 - Combustible Solids

WGK 3