InSolution Jasplakinolide, Jaspis johnstoni-Calbiochem

C₃₆H₄₅BrN₄O₆

709.67

A cell-permeable F-actin probe. This macrocyclic cyclodepsipeptide, isolated from the marine sponge Jaspis johnstoni, is a potent inducer of actin polymerization and stabilization in vitro. It competes with Phalloidin (Cat. No. 516640) for actin binding (K_d = 15 nM) and enhances apoptosis induced by interleukin 2 (IL-2) deprivation. Also displays fungicidal, insecticidal, and anticancer properties.

Primary Target
F-actin
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Kd = 15 nM for actin binding

Toxicity: Harmful (C)

A 1 mM (50 µg/71 µl) solution of Jasplakinolide, Jaspis johnstoni (Cat. No. 420107) in DMSO.

Following initial thaw, aliquot and freeze (-20°C).

Rosado, J.A., et al. 2000. J. Biol. Chem.275, 7527.
Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.
Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.
Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.
Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥90% (HPLC)

liquid

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

wet ice

−20℃

50 μg in Plastic ampoule
Packaged under inert gas