KAPA LongRange

NA.55

The KAPA HotStart^® ReadyMix^™ with dye Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA HotStart ReadyMix with dye Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.

KAPA LongRange HotStart^® ReadyMix^™ with dye has been used for:

• Amplification of long targets and/or low amounts
• Standard short- and mid-range polymerase chain reaction (PCR)
• T-A cloning
• Targeted sample enrichment for next-generation DNA sequencing
• Complete genome amplification

Both KAPA Taq HotStart^® DNA polymerase and a modified archaeal (Type B) DNA polymerase possess 5′→3′ polymerase activity, but only KAPA Taq HotStart DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA LongRange HotStart is 2–4 fold higher than that of Taq DNA Polymerase but is lower than the fidelity of pure proofreading polymerases such as KAPA HiFi. Reaction products generated with KAPA LongRange HotStart are polyA-tailed, and can be cloned into TA-cloning vectors.

Each batch of KAPA LongRange HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The 2X KAPA LongRange HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for longterm storage.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC

组份不可单独销售
KAPA Long Range DNA Polymerase or KAPA Long Range HotStart^® DNA Polymerase 5 U/μL or 2.5 U/μL
1X KAPA Long Range Buffer
dNTP Mix 0.3mM of each dNTP at 1X
2X KAPA LongRange HotStart^® ReadyMix with dye
MgCl₂ at 1X 2 mM

100

sufficient for 100 reactions
sufficient for 500 reactions

≤18 mo.

Long Range PCR
dNTPs included
hotstart

kit of 1.25 mL (100 x 25 μL rxn; KK3601)
kit of 6.25 mL (500 x 25 μL rxn; KK3602)

Roche

PCR: suitable

purified DNA

dry ice

−20℃

12 - Non Combustible Liquids

WGK 1