KAPA PROBE FAST

NA.55

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan^®), FRET probes, and displacement probes (e.g. molecular beacons).

KAPA PROBE FAST qPCR Master Mix (2X) with ROX^™ Low is suitable for use in:

• Genotyping
• Gene expression analysis
• Multiplex qPCR
• SNP genotyping/allelic discrimination
• Microarray validation
• Real time(RT)-quantitative polymerase chain reaction (qPCR)/qPCR

KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20℃ when not in use. When protected from light, reagents are stable in the dark at 4℃ for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

For Research Use Only. Not for use in diagnostic procedures.

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

组份不可单独销售
KAPA PROBE FAST qPCR Master Mix (2X) with ROX^™ Low
KAPA Taq HotStart^® DNA Polymerase
reaction buffer
dNTPs
MgCl₂ 5 nM

100

sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions

≤12 mo.

dNTPs included
hotstart

kit of 1 mL (100 x 20 μL rxn; KK4716)
kit of 10 mL (1000 x 20 μL rxn; KK4718)
kit of 5 mL (500 x 20 μL rxn; KK4717)
pkg of

Roche

2 ×

qPCR: suitable

purified DNA

for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems^® 7500
for use with Applied Biosystems^® ViiA 7
for use with QuantStudio^™

probe-based

wet ice

−20℃

12 - Non Combustible Liquids

WGK 1