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KAPA SYBR® FAST
| 产品编号： | 4157683 |
| 规格： | suitable for qPCR, 2 ×, Bio-Rad iCycler® |
| 包装规格： | 1 ML，5 ML，10 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Roche |
| 优惠价： | 立即咨询 |
基本信息
| NACRES | NA.55 |
| General description【一般描述】 | KAPA SYBR FAST qPCR Kits for Bio-Rad iCycler® contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity. |
| Application【应用】 | KAPA2G Fast HotStart® ReadyMix™ has been used for:
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| Features and Benefits【特点和优势】 | Quantitate changes in gene expression more accurately
Detect low copy and difficult targets consistently
Complete real-time PCR runs in just 40 minutes
Quick Notes:
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| Quality【质量】 | All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination. |
| Preparation Note【制备说明】 | Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20℃. The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) for Bio-Rad® iCycler® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20℃ when not in use. When protected from light, reagents are stable in the dark at 4℃ for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases. |
| Other Notes【其他说明】 | For Research Use Only. Not for use in diagnostic procedures. |
| Legal Information【法律信息】 | Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc. HOTSTART is a registered trademark of Molecular BioProducts, Inc. ReadyMix is a trademark of sigma-aldrich Co. LLC SYBR is a registered trademark of Life Technologies iCycler is a registered trademark of Bio-Rad |
| Components【组分】 | 组份不可单独销售 KAPA SYBR® FAST DNA Polymerase reaction buffer dNTPs SYBR® Green I dye MgCl2 2.5 mM |
产品性质
| Quality Level【质量水平】 | 100 |
| usage【用途】 | sufficient for 100 reactions sufficient for 1000 reactions sufficient for 500 reactions |
| shelf life【保质期】 | ≤12 mo. |
| feature【特点】 | dNTPs included hotstart: no |
| packaging【包装】 | kit of 1 mL (100 x 20 μL rxn; KK4606) kit of 10 mL (1000 x 20 μL rxn; KK4608) kit of 5 mL (500 x 20 μL rxn; KK4607) |
| manufacturer/tradename | Roche |
| concentration【浓度】 | 2 × |
| technique(s) | qPCR: suitable |
| input | purified DNA |
| compatibility【相容性】 | for use with Bio-Rad iCycler |
| Detection Method | SYBR® Green |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H371 |
| Precautionary Statements | P260 - P264 - P270 - P308 + P311 - P405 - P501 |
| Hazard Classifications【危险分类】 | STOT SE 2 |
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
