KAPA Taq 热启动

NA.55

KAPA Taq HotStart^® with dNTPs is supplied in a 2X ReadyMix^™ format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA Polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.

KAPA Taq HotStart^® has been used for:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Aquarium DNA extraction to generate marker gene libraries

KAPA Taq HotStart^® DNA polymerase and KAPA Taq exhibit 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The enzyme shows an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotstart buffer is formulated uniquely to facilitate specific primer annealing. This translates to higher yields of the specific product when compared to traditional Taq buffers. It also enhances the amplification of GC- and AT-rich templates. KAPA Taq hotstart DNA polymerase might be used in combination with any standard Taq buffer with a pH of 8.3 or higher

Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long term storage.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC

组份不可单独销售
KAPA Taq HotStart^® DNA Polymerase 5 U/µL
5X KAPA Taq HotStart^® Buffer
MgCl₂ 25 mM
KAPA dNTP Mix 10 mM each

100

sufficient for 1000 reactions
sufficient for 500 reactions

≤18 mo.

dNTPs included
hotstart

pkg of 250 U (KK1509)
pkg of 500 U (KK1511)

Roche

PCR: suitable

purified DNA

dry ice

−20℃

GHS07,GHS08

Warning

H302 - H371

P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501

Acute Tox. 4 Oral - STOT SE 2

12 - Non Combustible Liquids

WGK 1