KAPA Taq PCR 试剂盒

NA.56

KAPA Taq is supplied in a 2X ReadyMix^™ format. It comprises all the components required for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq DNA Polymerase comprises two reaction buffers (Buffer A and Buffer B) or a single buffer with loading dye. It aids convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers. KAPA Taq Buffer B is a Tris-potassium chloride buffer. KAPA Taq DNA Polymerase combines with any standard Taq buffer with a pH of 8.3 or higher.

KAPA Taq PCR Kit may be used in:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Polymorphism genotyping

KAPA Taq DNA Polymerase is a single-subunit, wild-type Taq DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities. It does not have 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq associates with a proprietary antibody and inactivates the enzyme until the first denaturation step. Hence, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long term storage.

For Research Use Only. Not for use in diagnostic procedures.

ReadyMix is a trademark of sigma-aldrich Co. LLC

组份不可单独销售
KAPA Taq DNA Polymerase 5 U/µL
10X KAPA Taq Buffer with loading dye
MgCl₂ 25 mM

100

≤18 mo.

dNTPs included: no
hotstart: no

pkg of 250 U (KK1020)
pkg of 2500 U (BK1004)
pkg of 500 U (KK1022)
pkg of 5000 U (BK1006)

Roche

PCR: suitable

purified DNA

dry ice

−20℃

12 - Non Combustible Liquids

WGK 1