含dNTP的KAPA Taq PCR试剂盒

NA.56

KAPA Taq is supplied in a 2X ReadyMix^™ format consisting all the components essential for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq DNA Polymerase is provided with two reaction buffers (Buffer A and Buffer B) or a single buffer with loading dye. It allows convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers, whereas KAPA Taq Buffer B is a Tris-potassium chloride buffer. KAPA Taq DNA Polymerase is used in combination with any standard Taq buffer with a pH of 8.3 or higher.

KAPA Taq PCR Kit with dNTPs may be used for:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Polymerase chain reaction (PCR)

KAPA Taq DNA Polymerase is a single-subunit, wild-type Taq DNA polymerase extracted from thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart^® DNA Polymerase shows 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The enzyme elicits error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In hot start formulation, the KAPA Taq combines with a proprietary antibody and inactivates the enzyme until the first denaturation step. It eliminates the spurious amplification of products and increases the reaction efficiency and sensitivity.

Each batch of KAPA Taq DNA Polymerase is confirmed tocontain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endo nuclease activity, and meet strict requirements with respect to DNA contamination levels.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ forshort-term use (up to 1 month). Return to -20℃ for long term storage.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC

组份不可单独销售
KAPA Taq DNA Polymerase 5 U/µL
10X KAPA Taq Buffer with loading dye
MgCl₂ 25 mM
KAPA dNTP Mix 10 mM each

100

≤18 mo.

dNTPs included
hotstart: no

pkg of 250 U (KK1021)
pkg of 2500 U (BK1005)
pkg of 500 U (KK1023)
pkg of 5000 U (BK1007)

Roche

PCR: suitable

purified DNA

dry ice

−20℃

12 - Non Combustible Liquids

WGK 1