MISSION^® Lenti microRNA, Human

NA.51

Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.

Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.

The microRNA hsa-miR-25-3p has been shown to have an elevated expression in osteosarcoma cells. It may be a biomarker for pancreatic cancer. miR-25-3p is also upregulated in several tumors and the gene encoding it is localized on human chromosome 7q22.1.

Based on miRBase V20 Mature ID

Two negative controls are available: NCLMIR001 and NCLMIR002

MISSION is a registered trademark of sigma-aldrich Co. LLC

200

MISSION^®

≥1x10⁶ VP/ml (via p24 assay)

CAUUGCACUUGUCUCGGUCUGA

MIMAT0000081

MI0000082

dry ice

−70℃

12 - Non Combustible Liquids

WGK 3