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MISSION® Lenti microRNA, Mouse
基本信息
NACRES | NA.51 |
General description【一般描述】 | Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells. |
Other Notes【其他说明】 | Based on miRBase V20 Mature ID |
Recommended products【推荐产品】 | Two negative controls are available: NCLMIR001 and NCLMIR002 |
Legal Information【法律信息】 | MISSION is a registered trademark of sigma-aldrich Co. LLC |
产品性质
Quality Level【质量水平】 | 200 |
product line【产品线】 | MISSION® |
concentration【浓度】 | ≥1x106 VP/ml (via p24 assay) |
mature sequence【成熟序列】 | UGUGCAAAUCUAUGCAAAACUGA |
Sanger mature/minor accession no.【Sanger mature/minor 登记号】 | MIMAT0000651 |
Sanger microRNA accession no.【Sanger microRNA登记号】 | MI0000688 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |