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MultiDsk-Ubiquitin-binding protein reagent
| 产品编号： | 4167814 |
| 规格： | This MultiDsk-Ubiquitin-binding protein reagent is validated for use in Affinity Precipitation Assays for the pull-down of mono- and poly-ubiquitinated proteins. |
| 包装规格： | 15 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
MultiDsk-Ubiquitin-binding protein reagent
Dsk-Ubiquitin-binding protein reagent, Ubiquitin domain-containing protein DSK2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | MultiDsk - Ubiquitin-binding protein reagent is a GST fusion protein containing an array of five ubiquitin-associated (UBA) domains from the yeast Ub-binding protein Dsk2 (aa 327-373; UniProt P48510). MultiDsk binds and protects ubiquitinated proteins from deubiquitination. When immobilized on glutathione resins, MultiDsk can be used for affinity pull-down of mono- and poly-ubiquitinated proteins from cell extracts and tissue homogenates. This construct also contains a C-terminal 6xHis sequence and an internal 6xHis sequence between the N-terminal GST and the 5XUBA sequence (PMID 23056298). MultiDsk is expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). Product Source: Yeast protein, with a GST tag and a His tag, expressed in e. coli. |
| Specificity【特异性】 | Mono- and poly-ubiquitinated proteins |
| Immunogen【免疫原】 | Epitope: Ubiquitin This is an protein reagent, not an antibody as it is a yeast protein, with a GST tag and a His tag, expressed in e. coli. |
| Application【应用】 | Affinity Precipitation Assay Analysis: A representative lot of MultiDsk-Ubiquitin-binding protein was immobilized on agarose beads and used to precipitate ubiquitinated proteins from yeast and HEK293 whole cell extracts. (Wilson, D.M., et al. (2012) PLoS One. 7(10):e46398). Affinity Precipitation Assay Analysis: A representative lot of MultiDsk-Ubiquitin-binding protein was immobilized on agarose beads and used to pull down ubiquitinated proteins from yeast whole cell extracts. (Wilson, D.M., et al. (2013) Cell. 154:983-995). |
| Quality【质量】 | Evaluated by Affinity Precipitation in yeast whole cell extract. Affinity Precipitation Assay Analysis: Ubiquitinated proteins were isolated from 1.0 mg yeast whole cell extract by MultiDsk-Ubiquitin-binding protein reagent (7.8 ug to 31.3 ug MultiDsk per pull-down) immobilized on agarose beads |
| Physical form【外形】 | Purified ubiquitin-binding protein in buffer containing 25mM Tris-HCl pH7.5, 150mM NaCl, 2mM β-mercaptoethanol with 50% glycerol without preservatives. Affinity Format: Purified |
| Analysis Note【分析说明】 | Multiple bands observed. |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | Saccharomyces cerevisiae |
| technique(s) | affinity precipitation assay: suitable |
| UniProt accession no.【UniProt登记号】 | P48510 |
| shipped in【运输】 | dry ice |
产品说明
| Storage and Stability【储存及稳定性】 | Stable for 1 year at -80°C from date of receipt. Shipped on Dry Ice. Upon delivery aliquot and store at -80°C. Avoid freeze / thaw cycle |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
