PCR 缓冲液不含 MgCl₂，10x 浓缩

solution

100

pkg of 3 × 1 mL

Roche

dry ice

−20℃

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

溶液，100 mM Tris-HCl，500 mM KCl (pH 8.3，20℃)

供一般实验室使用。

12 - Non Combustible Liquids

WGK 1