Rho Kinase Inhibitor III, Rockout-CAS 7272-84-6-Calbiochem

C₁₃H₁₀N₂

194.23

A cell-permeable indolopyridine compound that acts as a selective, reversible, and ATP-competitive inhibitor of Rho kinase activity with an IC₅₀ of 25 µM. Does not inhibit the activation of Rho kinase nor does it affect the in vitro activities of MLCK, PKCα, and SAPK2a/p38α. Shown to be 5-fold less potent than Y-27632 (Cat. No. 688000; IC₅₀ = ~5 µM), and display a similar specificity profile as H-89 (Cat. No. 371963). Affects cell migration, inhibits blebbing (IC₅₀ = ~ 12 µM in M2 cells), and decreases stress fibers in Bulb 3T3 cells at 50 µM.
A cell-permeable, selective, reversible, and ATP-competitive inhibitor of Rho kinase activity (IC₅₀ = 25 µM). Does not inhibit the activation of Rho kinase nor does it affect the in vitro activities of MLCK, PKCα, and SAPK2a/p38α. Shown to be 5-fold less potent than Y-27632 (Cat. No. 688000; IC₅₀ ~5 µM), and display a similar specificity profile as H-89 (Cat. No. 371963). Affects cell migration, inhibits blebbing (IC₅₀ ~12 µM in M2 cells), and decreases stress fibers in Bulb 3T3 cells at 50 µM.

Cell permeable: yes
Primary Target
ROCK
Target IC₅₀: 25 µM against ROCK
Product competes with ATP.
Reversible: yes

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Yarrow, J.C., et al. 2005. Chem. Biol.12, 385.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

yellow

DMSO: 25 mg/mL
methanol: soluble

ambient

2-8℃

1S/C13H10N2/c1-2-4-13-11(3-1)12(9-15-13)10-5-7-14-8-6-10/h1-9,15H

LLJRXVHJOJRCSM-UHFFFAOYSA-N

10 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1