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STBCi004-A

产品编号:4169292
规格:Human iPS Cell Line
包装规格:1 VIAL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41692921 VIAL11450
产品性质
biological source【生物来源】
human dermis (fibroblast)
reprogramming method【重编程方法】
Sendai virus
description【描述】
age (N/A)
manufacturer/tradename
EBiSC
gender【性别】
female
growth mode【生长模式】
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)【相关疾病】
Parkinson′s disease
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
General description【一般描述】
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin【细胞系来源】
Depositor
StemBANCC
Cell Line Description【细胞系描述】
Derivation
Primary cell type: Fibroblast
Tissue collection year: 2012
Passage number reprogrammed: 4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes -
TRA 1-60 Yes -
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 20
Cell line karyotype: No abnormalities were detected
Karyotyping method: Molecular karyotyping by SNP array
Image / Result

STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Linkage【联系】
  • Additional Cell Line Info
  • EBiSC Access Use Agreement (EAUA)
  • Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment
Subculture Routine【传代培养常规】
Medium: mTeSR®1
Passage method: EDTA
Matrix: Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37℃
Legal Information【法律信息】
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
mTeSR is a registered trademark of WiCell Research Institute, Inc.
安全信息
Storage Class Code【储存分类代码】
6.2 - Infectious substances
WGK
WGK 3
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