TNF-α, Mouse, Recombinant, E. coli

100

≥97% (SDS-PAGE)

lyophilized

Calbiochem^®

OK to freeze

≤100 pg/μg Endotoxin (pg/μg TNF-α)

ambient

−70℃

10 μg in Plastic ampoule

Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. It is referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune system′s response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.

ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

Toxicity: Standard Handling (A)

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.

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11 - Combustible Solids

WGK 1