Wee1, GST-Fusion, Human, Recombinant, S. frugiperda

2.7.1.112 ( BRENDA | IUBMB )

Recombinant, human WEE1 fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 µM).

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Following initial thaw, aliquot and freeze (-70°C).

Katayama, K., et al. 2005. Mol. Cell. Biol.25, 5725.
Yoshida, T., et al. 2004. Ann. Oncol.15, 252.
Watanabe, N., et al. 1995. EMBO J.14, 1878.
Igarashi, M., et al. 1991. Nature353, 80.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

liquid

100

≥1 units/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

10 - Combustible liquids

WGK 1