脂解(脂肪细胞)检测试剂盒

259-322-7

NA.25

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.

The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λ_ex = 535/ λ_em = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.

组份不可单独销售
Collagenase (0.2%)
Collagenase Stop Buffer
Adipocyte Wash Buffer
Adipocyte Lipolysis Buffer
Glycerol Assay Buffer
Glycerol Probe, in DMSO
Glycerol Enzyme Mix
Glycerol Standard, 100 mM
Isoproterenol, 10 mM
Cell Strainer

colorimetric
fluorometric

−20℃

该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。

GHS07

Warning

H315 - H319 - H335

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Respiratory system

10 - Combustible liquids

WGK 3