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SYBR^® Green Extract-N-Amp^TM组织PCR试剂盒

SYBR^® Green Extract-N-Amp^TM Tissue PCR Kit

产品编号：

3246201

产品类别：

试剂盒

纯度规格：

sufficient for 100 extractions, sufficient for 100 amplifications

包装规格：

1 KIT

市场价：

4500元

优惠价：

立即咨询

SYBR^® Green Extract-N-Amp^TM组织PCR试剂盒

SYBR^® Green Extract-N-Amp^TM Tissue PCR Kit

产品性质

usage【用途】	sufficient for 100 amplifications sufficient for 100 extractions sufficient for 100 reactions
Quality Level【质量水平】	200
feature【特点】	dNTPs included hotstart
technique(s)	PCR: suitable
web metadata keyword.default	XNATRG
storage temp.【储存温度】	−20℃

基本信息

General description【一般描述】	The SYBR^® Green Extract-N-Amp^™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Application【应用】	Extract-N-Amp Video Protocol SYBR^® Green Extract-N-Amp^™ tissue PCR kit has been used for DNA extraction from embryo head of male Pmp22 transgenic Sprague–Dawley rats and in genotyping. This direct PCR (polymerase chain reaction) kit is also suitable for gene copy number experiments and amplifying and quantifying DNA from multiple tissue sample types.
Features and Benefits【特点和优势】	Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR) Fast – tissue to qPCR in 15 minutes Convenient – no long enzymatic digestions and no column purifications Simple – rapid, easy-to-follow protocol Sensitive – specially formulated Hot Start^® SYBR^® Green PCR ReadyMix^™ for highly specific PCR amplification and quantitation of genomic DNA Safe – no organic extraction with hazardous chemicals
Components【组分】	试剂盒含有Extraction Solution、Neutralization Solution、Tissue Preparation Solution和Extract-N-Amp SYBR Green PCR ReadyMix^™。Extract-N-Amp SYBR Green PCR ReadyMix为2X反应混合物，其中含有 SYBR Green、缓冲液、盐、dNTP、Taq聚合酶和 JumpStart^™ Taq 抗体。此试剂盒专为结合提取试剂使用进行了优化，其中含有适用于热启动PCR、可增强特异性的 JumpStart Taq抗体，以及可用作实时定量PCR的非特异性报告标志物的SYBR Green I。
Principle【原理】	DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp^™ SYBR^® Green PCR ReadyMix^™ and user-provided PCR primers.
Other Notes【其他说明】	更多信息，请见www.sigma-aldrich.com/extract-n-amp。
Legal Information【法律信息】	本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056和6,171,785。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。其他专利声明下的任何权利(如美国专利号6,814,934号下的设备和系统专利声明)、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。 Extract-N-Amp is a trademark of sigma-aldrich Co. LLC HOTSTART is a registered trademark of Molecular BioProducts, Inc. JumpStart is a trademark of sigma-aldrich Co. LLC ReadyMix is a trademark of sigma-aldrich Co. LLC SYBR is a registered trademark of Life Technologies

安全信息

Pictograms【象形图】	GHS07,GHS08,GHS09
Signal word【警示用语：】	Danger
Hazard Statements	H315 - H317 - H319 - H334 - H335 - H411
Precautionary Statements	P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】	Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs【靶器官】	Respiratory system
Storage Class Code【储存分类代码】	10 - Combustible liquids
WGK	WGK 3

SYBR® Green Extract-N-AmpTM组织PCR试剂盒

SYBR^® Green Extract-N-Amp^TM组织PCR试剂盒