来自 荆豆的凝集素

NA.32

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

荆豆凝集素具有抗H血型特异性。凝集素有两种类型： UEA I对 L-岩藻糖具有亲和力，UEA II对N,N′-二乙酰基壳二糖具有亲和力。最初认为UEA I摩尔量为170,000道尔顿。 后来的报道表明，UEA I可以在中性和碱性pH下形成聚集体，正确的摩尔量是68,000道尔顿。

由亲和纯化凝集素制备偶联物。

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 ℃.

Contains sodium citrate buffer salts

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

凝集活性以μg/ml表示，由磷酸盐缓冲液(pH 6.8)连续稀释1 mg/ml溶液而测得。该活性是指25℃孵育1小时后使2％人O型血红细胞悬浮液凝集的最低浓度。

200

peroxidase conjugate

70.00-90.00% (Lowry)

lyophilized powder

<40 μg/mL agglutination activity

≥15 units/mg protein

Protein, ~80% Lowry

western blot: suitable

2-8℃

0.5, 1 mg in serum bottle
Package size based on protein content.

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)