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产品性质
| biological source【生物来源】 | mouse liver |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Epithelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Mouse C3H/An liver |
| Cell Line Description【细胞系描述】 | Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain). |
| Application【应用】 | Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana) |
| Culture Medium【培养基】 | NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS) |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37℃. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
