PCSK9 murine

NA.32

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

mouse

expressed in HEK 293 cells

6-His tagged (N-terminus)

≥97% (SDS-PAGE)

lyophilized powder

calculated mol wt 72 kDa
observed mol wt 20 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 35 is the predicted N-terminus .)
observed mol wt 64 kDa

pkg of 20 μg

<1 EU/μg endotoxin (LAL test)

Q80W65

wet ice

−20℃

mouse ... PCSK9(100102)

11 - Combustible Solids

WGK 3