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产品性质
| biological source【生物来源】 | human colon |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 46, triploid |
| morphology【形态学】 | Epithelial |
| products【产品】 | Carcinoembryonic antigen (CEA) |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human Caucasian colon adenocarcinoma |
| Cell Line Description【细胞系描述】 | Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus. |
| Application【应用】 | Tumourigenicity studies |
| Culture Medium【培养基】 | L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37℃. Cells do not reach confluency but will grow in small islands. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
