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产品性质
| biological source【生物来源】 | hamster lung |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 22 |
| morphology【形态学】 | Fibroblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Hamster Chinese lung |
| Cell Line Description【细胞系描述】 | Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102). |
| Application【应用】 | Mutagenesis and mammalian cell genetic studies |
| Culture Medium【培养基】 | DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm2; 5% CO2; 37℃ using 0.25% trypsin/EDTA. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
