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产品性质
| biological source【生物来源】 | human placenta |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high. |
| morphology【形态学】 | Epithelial |
| products【产品】 | At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase. |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human placenta, SV40 transformed |
| Cell Line Description【细胞系描述】 | Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage. GMO1 |
| Culture Medium【培养基】 | α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 36℃. Change medium 2-3 times weekly. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
