α-甘油磷酸脱氢酶 来源于兔肌肉

1.1.1.8 ( BRENDA | IUBMB )

232-982-3

MFCD00130487

NA.54

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

• in the reaction mixture to measure the glycerol kinase activity
• to demonstrate compartmentalized enzymatic reactions where NADH is involved
• in the reaction mixture assay for L-fuculose-1-phosphate aldolase

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

在 pH 7.4，25℃ 下，一单位酶每分钟将 1.0 μmole 磷酸二羟丙酮转化为 α-甘油磷酸酯。

溶于 3.2M(NH₄)₂SO₄ 和 0.1g/L EDTA 溶液中的结晶悬浮液，pH 6.0

根据改良的 Fondy 法进行制备。

缩二脲法测定蛋白

200

Type I

ammonium sulfate suspension

100-300 units/mg protein

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%

2-8℃

12 - Non Combustible Liquids

WGK 2