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Carboxy-terminal FLAG-BAPTM Fusion Protein
基本信息
| MDL number | MFCD00130921 |
| NACRES | NA.32 |
| General description【一般描述】 | Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP). |
| Application【应用】 | Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF). |
| Biochem/physiol Actions【生化/生理作用】 | The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay. |
| Other Notes【其他说明】 | Control protein |
| Physical form【外形】 | Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2 |
| Preparation Note【制备说明】 | Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml |
| Legal Information【法律信息】 | FLAG-BAP is a trademark of sigma-aldrich Co. LLC |
产品性质
| form【形式】 | liquid |
| Quality Level【质量水平】 | 200 |
| mol wt【分子量】 | ~49 kDa |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
