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Topo I (C651-765) (C terminal domain) human
| 产品编号： | 4084965 |
| 规格： | recombinant, expressed in insect cells, ≥80% (SDS-PAGE) |
| 包装规格： | 5 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Topo I (C651-765) (C terminal domain) human
TOPI
基本信息
| NACRES | NA.26 |
| Biochem/physiol Actions【生化/生理作用】 | Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA. |
| Physical form【外形】 | Clear and colorless frozen liquid solution |
| Preparation Note【制备说明】 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in insect cells |
| Assay【测定】 | ≥80% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| mol wt【分子量】 | ~15.2 kDa |
| packaging【包装】 | pkg of 5 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | 500 μg/mL |
| color【颜色】 | clear colorless |
| NCBI accession no.【NCBI登记号】 | NM_003286 |
| UniProt accession no.【UniProt登记号】 | P11387 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... TOP1(7150) |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
