DAF-FM DA-CAS 254109-22-3-Calbiochem

C₂₅H₁₈F₂N₂O₇

496.42

MFCD16872050

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Primary Target
Nitric oxide (NO) fluorescent indicator

Toxicity: Irritant (B)

A 5 mM solution in DMSO.

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solution

Calbiochem^®

OK to freeze
protect from light

pale yellow

λ_ex ~500  nm
λ_em ~515 nm

−20℃

10 - Combustible liquids

WGK 2

188.6 °F - (Dimethylsulfoxide)

87 ℃ - (Dimethylsulfoxide)