Caspase-10, Human, Recombinant, E. coli

100

≥95% (SDS-PAGE)

liquid

≥150,000 units/mg protein
≥50,000 units/mL

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

3000 u in Plastic ampoule

M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.

Following initial thaw, aliquot and freeze (-70°C).

Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 2