CyclinE1, GST tagged human

NA.32

Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle that functions as regulators of CDK kinases. Cyclin E1 activity is required for centrosome duplication during S phase and this mechanism could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis. The downregulation of cyclin E-CDK2 kinase activity following the G1/S-phase transition that is necessary for the maintenance of karyotypic stability. Cyclin E also has a modular centrosomal-targeting domain which is essential for promoting S phase entry in a Cdk2-independent manner.

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

After opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles.

expressed in baculovirus infected Sf9 cells

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

~73 kDa

NM_001238

dry ice

−70℃

human ... CCNE1(898)

10 - Combustible liquids

WGK 1