Angiotensin Converting Enzyme-2, His·Tag^®, Human, Recombinant, NSO Cells

100

expressed in NSO cells

≥90% (SDS-PAGE)

liquid

≥0.8 units/μg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

≤1.0 EU/μg Endotoxins (EU/μg ACE-2)

wet ice

−70℃

10 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. ACE-2 cleaves angiotensins I and II as a carboxypeptidase and plays an important role in regulating cardiovascular and renal functions. Native ACE-2 is an integral membrane zinc metalloprotease whose activity is inhibited by EDTA. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing conditions.
Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Native ACE-2 is an integral membrane zinc metalloprotease that can be inhibited by EDTA. ACE-2 cleaves angiotensins I and II. Plays an important role in regulating cardiovascular and renal functions. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing condition.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-YVADAPK(DNP)-OH per min at 25°C, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Tikellis, C., et al. 2003. Hypertension41, 390.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1